Chèvre anti-Lapin IgG (Fc Region) Anticorps (DyLight 680) - Preadsorbed

N° du produit ABIN6699129

Détail du produit

Antigène: IgG

Épitope: Fc Region

Reactivité: Lapin

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: DyLight 680

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Attributs du produit:

Synonyms: Goat Anti Rabbit IgG F(c) DyLight 680™ Conjugated Antibody, Goat Anti-Rabbit IgG Fc Fragment Antibody DyLight 680™ conjugation, Goat Anti Rabbit IgG Fc Antibody DyLight 680™ conjugated

Background: Anti-Rabbit IgG F(c) DyLight generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.

Purification: Preadsorption: Solid phase absorption

Immunogène:

Immunogen: Rabbit IgG F(c) fragment

Isotype: IgG

Labeling Ratio: 2.8

Information d'application

Indications d'application:

Application Note: The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Référence

Meese, Cepeda, Gahlen, Adams, Ficner, Ricci, Heller, Reisinger, Herget: "Activity-Dependent Phosphorylation by CaMKIIδ Alters the Ca2+ Affinity of the Multi-C2-Domain Protein Otoferlin." dans: Frontiers in synaptic neuroscience, Vol. 9, pp. 13, (2017) (PubMed).

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody