Chèvre anti-Rat IgM (Chain mu) Anticorps (DyLight 800) - Preadsorbed
-
- Antigène Tous les produits IgM
- IgM
-
Épitope
- Chain mu
-
Reactivité
- Rat
-
Hôte
- Chèvre
-
Clonalité
- Polyclonal
-
Conjugué
- DyLight 800
-
Application
- Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- Attributs du produit
-
Synonyms: Goat Anti Rat IgM (mu chain) Antibody DyLight 800™ Conjugated, Goat Anti-Rat IgM mu chain Antibody DyLight 800™ Conjugation
Background: Anti-Rat IgM antibody specifically detects rat IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Rat IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology. This Rat IgM Antibody is conjugated to DyLight™800.
- Purification
- Preadsorption: Solid phase absorption
- Immunogène
-
Immunogen: Rat IgM whole molecule
- Isotype
- IgG
- Labeling Ratio
- 2.0
-
-
- Indications d'application
-
Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
FLISA Dilution: >1:20,000
Western Blot Dilution: >1:10,000
IF Microscopy Dilution: >1:5,000
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01 % (w/v) Sodium Azide - Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- RT,4 °C,-20 °C
- Stockage commentaire
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Date de péremption
- 12 months
-
- Antigène
- IgM
- Abstract
- IgM Produits
- Classe de substances
- Antibody
-