Recombinant Alpaca anti-Souris IgG Anticorps (Alexa Fluor 568)

N° du produit ABIN7272914

Détail du produit

Antigène: IgG

Type d'anticorp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivité: Souris

Hôte: Alpaca

Expression System: E.coli

Clonalité: Monoclonal

Conjugué: Alexa Fluor 568

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificité: Mouse IgG (Kappa light chain), does not cross-react with rabbit, guinea pig or chicken immunoglobulins

Purification: Produced in: E.coli

Immunogène: Our sdAbs anti-mouse KLC specifically binds to all mouse Ig isotypes carrying a kappa light chain.

Clone: 1A23

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Information d'application

Indications d'application: IF: 1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed bellow)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM

Buffer: The single sdAb clone was lyophilized from PBS pH 7.4.

Stock: 4 °C/-20 °C/-80 °C

Stockage commentaire: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet: In most research monoclonal antibodies are generated in mice. These animals have a rich repertoire of immunoglobulins (Ig) displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mice IgGs can be further categorized into five subclasses IgG1, IgG2a, IgG2b, IgG2c, and IgG3. In addition to the subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain.