ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)
N° du produit (Fournisseur)
611-1302-1.0
Fournisseur
Rockland
Fonction
Goat Anti-Rabbit IgG H&L (HRP)
Specificité
IgG (H&L)
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Anti-Rabbit IgG Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Restore with deionized water (or equivalent)
Concentration
2.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Gentamicin Sulfate, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Agent conservateur
Gentamicin sulfate
Précaution d'utilisation
This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Stock
4 °C,-20 °C
Stockage commentaire
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Date de péremption
12 months
Mochizuki, Okumo, Takemura, Izukashi, Tatsuo, Ikemoto, Adachi, Kawate, Sunagawa: "Suppressive Activity of Boiogito, a Japanese Traditional Kampo Medicine, on Periostin Secretion in Human Fibroblast-Like Synoviocytes In Vitro." dans: Cureus, Vol. 16, Issue 4, pp. e57690, (2024) (PubMed).
Guo, Sperber, Krieger, Duan, Chemelewski, Sacchettini, Herman: "Bacillus subtilis YisK possesses oxaloacetate decarboxylase activity and exhibits Mbl-dependent localization." dans: Journal of bacteriology, Vol. 206, Issue 1, pp. e0020223, (2024) (PubMed).
Traczyk, Hromada-Judycka, Świątkowska, Wiśniewska, Ciesielska, Kwiatkowska: "Diacylglycerol kinase-ε is S-palmitoylated on cysteine in the cytoplasmic end of its N-terminal transmembrane fragment." dans: Journal of lipid research, Vol. 65, Issue 1, pp. 100480, (2024) (PubMed).
van Soest, Polderman, den Toom, Keijer, van Roosmalen, Leyten, Lehmann, Zwakenberg, De Henau, van Boxtel, Burgering, Dansen: "Mitochondrial H2O2 release does not directly cause damage to chromosomal DNA." dans: Nature communications, Vol. 15, Issue 1, pp. 2725, (2024) (PubMed).
Kücük, Abbey, Schmitt, Henninger, Fritz: "Cardiomyocytes, cardiac endothelial cells and fibroblasts contribute to anthracycline-induced cardiac injury through RAS-homologous small GTPases RAC1 and CDC42." dans: Pharmacological research, Vol. 203, pp. 107165, (2024) (PubMed).
Schröder, Wagner, Cieplik, Spanier, Proff, Kirschneck: "Impact of phosphorylation of heat shock protein 27 on the expression profile of periodontal ligament fibroblasts during mechanical strain." dans: Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie, Vol. 84, Issue Suppl 2, pp. 143-153, (2023) (PubMed).
Dai, Park, Du, Na, Lam, Chow, Renauer, Gu, Xin, Chu, Liao, Clark, Zhao, Slavoff, Chen: "Massively parallel knock-in engineering of human T cells." dans: Nature biotechnology, Vol. 41, Issue 9, pp. 1239-1255, (2023) (PubMed).
Arczewska-Włosek, Świątkiewicz, Tomaszewska, Muszyński, Dobrowolski, Józefiak: "Effects of Anticoccidial Vaccination and Taraxacum officinale Extract on the Growth Performance, Biochemical Parameters, Immunity, and Intestinal Morphology of Eimeria-Challenged Chickens." dans: Life (Basel, Switzerland), Vol. 13, Issue 9, (2023) (PubMed).
Bunduc, Ding, Kuijl, Marlovits, Bitter, Houben: "Reconstitution of a minimal ESX-5 type VII secretion system suggests a role for PPE proteins in the outer membrane transport of proteins." dans: mSphere, Vol. 8, Issue 5, pp. e0040223, (2023) (PubMed).
Park, Miyamoto, Han, Chan, Curnutt, Tran, Velleca, Kim, Schurer, Chang, Xu, Kharas, Woo: "Dual IKZF2 and CK1α degrader targets acute myeloid leukemia cells." dans: Cancer cell, Vol. 41, Issue 4, pp. 726-739.e11, (2023) (PubMed).
Castro, Umana-Perez, Castaño-Moreno, Casanello, Ronco: "DHA Supplementation during Pregnancy in Women with Obesity Normalizes IGF2R Levels in the Placenta of Male Newborns." dans: International journal of endocrinology, Vol. 2023, pp. 1515033, (2023) (PubMed).
Chen, Su, Zhao, Na, Jiang, Bacchiocchi, Loh, Halaban, Wang, Cao, Slavoff: "Unannotated microprotein EMBOW regulates the interactome and chromatin and mitotic functions of WDR5." dans: Cell reports, Vol. 42, Issue 9, pp. 113145, (2023) (PubMed).
Liu, Samant, Vasa, Pedrigi, Oguz, Ryu, Wei, Anderson, Agrawal, Chatzizisis: "Co-culture models of endothelial cells, macrophages, and vascular smooth muscle cells for the study of the natural history of atherosclerosis." dans: PloS one, Vol. 18, Issue 1, pp. e0280385, (2023) (PubMed).
Bina, Hines, Sanyal, Taneyhill: "Neurogenin 2 and Neuronal Differentiation 1 Control Proper Development of the Chick Trigeminal Ganglion and Its Nerve Branches." dans: Journal of developmental biology, Vol. 11, Issue 1, (2023) (PubMed).
Halmi, Wu, Taneyhill: "Neural crest cell-placodal neuron interactions are mediated by Cadherin-7 and N-cadherin during early chick trigeminal ganglion assembly." dans: F1000Research, Vol. 11, pp. 741, (2023) (PubMed).
Küchler, Teodoro, Schröder, Nazet, Meger, Kunz, Baratto-Filho, Spanier, Scariot, Proff, Kirschneck: "Effect of genetic polymorphisms rs2301113 and rs2057482 in the expression of HIF-1α protein in periodontal ligament fibroblasts subjected to compressive force." dans: Journal of applied oral science : revista FOB, Vol. 31, pp. e20220151, (2023) (PubMed).
Timón-Gómez, Pérez-Pérez, Nyvltova, Ugalde, Fontanesi, Barrientos: "Protocol for the Analysis of Yeast and Human Mitochondrial Respiratory Chain Complexes and Supercomplexes by Blue Native Electrophoresis." dans: STAR protocols, Vol. 1, Issue 2, (2022) (PubMed).
Ramirez, Yang, DSouza, Shen, Woo: "Truncation of the TPR domain of OGT alters substrate and glycosite selection." dans: Analytical and bioanalytical chemistry, Vol. 413, Issue 30, pp. 7385-7399, (2022) (PubMed).
Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1