ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)
N° du produit (Fournisseur)
609-4302-1.0
Fournisseur
Rockland
Fonction
Rabbit Anti-Human IgG H&L (HRP)
Specificité
IgG (H&L)
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG and Human Serum.
Classe de qualité
High-quality product from Rockland
Immunogène
Anti-Human IgG whole molecule was produced by repeated immunization with Human IgG whole molecule in rabbit. Immunogen Type: Native Protein
Anti-Human IgG (H&L) Peroxidase Conjugated Antibody has been tested by ELISA and Dot Blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Restore with deionized water (or equivalent)
Concentration
2.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Gentamicin Sulfate, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Agent conservateur
Gentamicin sulfate
Précaution d'utilisation
This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Stock
4 °C,-20 °C
Stockage commentaire
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1