ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Dot Blot (DB), Multiplex Assay (MA)
N° du produit (Fournisseur)
610-1202-1.0
Fournisseur
Rockland
Fonction
Goat Anti-Mouse IgG H&L (FITC)
Specificité
IgG (H&L)
Purification
Goat Anti-Mouse IgG [H&L] FITC Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG and Mouse Serum.
ELISA, IHC, WB
Polyclonal
IgG
unconjugated
Fab fragment
Indications d'application
Anti-Mouse IgG FITC Conjugated Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, flow cytometry, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Restore with deionized water (or equivalent)
Concentration
2.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide, 0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Date de péremption
12 months
Liu, Panagopoulos, Oguz, Samant, Vasa, Agrawal, Chatzizisis: "Role of triggering receptor expressed on myeloid cells-1 in the mechanotransduction signaling pathways that link low shear stress with inflammation." dans: Scientific reports, Vol. 13, Issue 1, pp. 4656, (2023) (PubMed).
Liu, Samant, Vasa, Pedrigi, Oguz, Ryu, Wei, Anderson, Agrawal, Chatzizisis: "Co-culture models of endothelial cells, macrophages, and vascular smooth muscle cells for the study of the natural history of atherosclerosis." dans: PloS one, Vol. 18, Issue 1, pp. e0280385, (2023) (PubMed).
Sundaramurthy, Votra, Laszlo, Davies, Pruyne: "FHOD-1 is the only formin in Caenorhabditis elegans that promotes striated muscle growth and Z-line organization in a cell autonomous manner." dans: Cytoskeleton (Hoboken, N.J.), Vol. 77, Issue 10, pp. 422-441, (2021) (PubMed).
Wang, Chen, Li, Zhang, Guo, Hu, Shao, Song, Zhao, Li, Yang, Xu, Wei: "Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor." dans: Redox biology, Vol. 32, pp. 101514, (2021) (PubMed).
Saferali, Tang, Strug, Quon, Zlosnik, Sandford, Turvey: "Immunomodulatory function of the cystic fibrosis modifier gene BPIFA1." dans: PloS one, Vol. 15, Issue 1, pp. e0227067, (2020) (PubMed).
Kolijn, Kovács, Herwig, Lódi, Sieme, Alhaj, Sandner, Papp, Reusch, Haldenwang, Falcão-Pires, Linke, Jaquet, Van Linthout, Mügge, Tschöpe, Hamdani: "Enhanced Cardiomyocyte Function in Hypertensive Rats With Diastolic Dysfunction and Human Heart Failure Patients After Acute Treatment With Soluble Guanylyl Cyclase (sGC) Activator." dans: Frontiers in physiology, Vol. 11, pp. 345, (2020) (PubMed).
Kryszke, Adjeriou, Liang, Chen, Dautry: "Post-transcriptional gene silencing activity of human GIGYF2." dans: Biochemical and biophysical research communications, Vol. 475, Issue 3, pp. 289-94, (2017) (PubMed).
Vitacolonna, Belharazem, Hohenberger, Roessner: "In-vivo quantification of the revascularization of a human acellular dermis seeded with EPCs and MSCs in co-culture with fibroblasts and pericytes in the dorsal chamber model in pre-irradiated tissue." dans: Cell and tissue banking, Vol. 18, Issue 1, pp. 27-43, (2017) (PubMed).
Adefolaju, Theron, Hosie: "BAX/BCL-2 mRNA and protein expression in human breast MCF-7 cells exposed to drug vehicles-methanol and dimethyl sulfoxide (DMSO) for 24 hrs." dans: Nigerian medical journal : journal of the Nigeria Medical Association, Vol. 56, Issue 3, pp. 169-74, (2015) (PubMed).
Cavaglia, Dombrowski, Drazba, Vasanji, Bokesch, Janigro: "Regional variation in brain capillary density and vascular response to ischemia." dans: Brain research, Vol. 910, Issue 1-2, pp. 81-93, (2001) (PubMed).
Bradley, Haskins, La Rosa, Lafferty: "CD8 T cells are not required for islet destruction induced by a CD4+ islet-specific T-cell clone." dans: Diabetes, Vol. 41, Issue 12, pp. 1603-8, (1992) (PubMed).
Smith, Pennline, Bober, Kenworthy-Bott, Phillips, Pellerito, da Fonseca, Terminelli: "Inhibition of collagen II-induced arthritis in mice--a comparison of the effects of Sch 24937, immunosuppressants and nonsteroidal antiinflammatory drugs on the clinical expression of disease." dans: International journal of immunopharmacology, Vol. 12, Issue 2, pp. 165-73, (1990) (PubMed).
Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1