Souris anti-Humain IgE Anticorps

N° du produit ABIN94398

Détail du produit

Antigène: IgE

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Application: ELISA, Flow Cytometry (FACS)

Specificité: The mouse monoclonal antibody 4G7.325 reacts with human IgE, it recognizes an epitope different from the ones recognized by BE5 and 4H10 antibodies to IgE. The epitope is located within the amino acids 103-115 (WSDYNFDYSSSEE).

Réactivité croisée (Details): Human

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Purified human IgE.

Clone: 4G7-325

Isotype: IgG

Information d'application

Indications d'application: Flow cytrometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Référence

Smith, Benjamin, Hozic, Derewenda, Smith, Thomas, Gafvelin, van Hage-Hamsten, Chapman: "The molecular basis of antigenic cross-reactivity between the group 2 mite allergens." dans: The Journal of allergy and clinical immunology, Vol. 107, Issue 6, pp. 977-84, (2001) (PubMed).

Mueller, Smith, Chapman, Rule, Benjamin: "Hydrogen exchange nuclear magnetic resonance spectroscopy mapping of antibody epitopes on the house dust mite allergen Der p 2." dans: The Journal of biological chemistry, Vol. 276, Issue 12, pp. 9359-65, (2001) (PubMed).

Détail du antigène

Antigène: IgE

Abstract: IgE Produits

Sujet: Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E

Poids moléculaire: 180 kDa