Lapin anti-Cobaye IgG (F(ab')2 Region) Anticorps
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- Antigène Tous les produits IgG
- IgG
- Fragment
- F(ab')2 fragment
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Épitope
- F(ab')2 Region
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Reactivité
- Cobaye
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificité
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Guinea Pig IgG, Guinea Pig IgG F(ab')2 and Guinea Pig Serum.
- Purification
- This product is a F(ab')2 fragment of IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Guinea Pig IgG, Guinea Pig IgG F(ab')2 and Guinea Pig Serum. No reaction was observed against Guinea Pig IgG F(c), anti-Rabbit IgG F(c) or anti-Pepsin.
- Immunogène
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Immunogen: Guinea Pig IgG F(ab')2 fragment
- Isotype
- IgG
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- Indications d'application
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Immunohistochemistry Dilution: 1:1,000-1:5,000
Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
ELISA Dilution: 1:20,000-1:100,000
Western Blot Dilution: 1:2,000-1:10,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- Conseil sur la manipulation
- Centrifuge product if not completely clear after standing at room temperature.
- Stock
- RT,4 °C,-20 °C
- Stockage commentaire
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
- Date de péremption
- 12 months
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- Antigène
- IgG
- Abstract
- IgG Produits
- Classe de substances
- Antibody
- Sujet
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Synonyms: rabbit F(ab')2 Anti-GUINEA PIG IgG F(ab')2 fragment Antibody, rabbit F(ab')2 Anti-GUINEA PIG IgG Fab2 fragment Antibody, rabbit Fab2 Anti Guinea Pig IgG Fab2
Background: F(ab')2 Anti-Guinea Pig IgG F(ab')2 Antibody generated in rabbit detects Guinea Pig F(ab')2. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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