Chèvre anti-Souris IgG (Heavy & Light Chain) Anticorps (HRP) - Preadsorbed

N° du produit ABIN964951

Détail du produit

Antigène: IgG

Épitope: Heavy & Light Chain

Reactivité: Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)

Specificité: IgG (H&L)

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: immunoaffinity chromatography using Mouse IgG coupled to agarose beads

Immunogène: Mouse IgG whole molecule

Isotype: IgG

Information d'application

Indications d'application: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 2.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Gentamicin sulfate

Conseil sur la manipulation: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Référence

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Hashimoto, Pais, Zhang, Saleh, Fu, Dai, Corrêa, Zheng, Cheng: "Structure of a Naegleria Tet-like dioxygenase in complex with 5-methylcytosine DNA." dans: Nature, Vol. 506, Issue 7488, pp. 391-5, (2014) (PubMed).

Frasca, Romero, Diaz, Alter-Wolf, Ratliff, Landin, Riley, Blomberg: "A molecular mechanism for TNF-α-mediated downregulation of B cell responses." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 1, pp. 279-86, (2012) (PubMed).

Frasca, Romero, Landin, Diaz, Riley, Blomberg: "Protein phosphatase 2A (PP2A) is increased in old murine B cells and mediates p38 MAPK/tristetraprolin dephosphorylation and E47 mRNA instability." dans: Mechanisms of ageing and development, Vol. 131, Issue 5, pp. 306-14, (2010) (PubMed).

Ronsyn, Daans, Spaepen, Chatterjee, Vermeulen, DHaese, Van Tendeloo, Van Marck, Ysebaert, Berneman, Jorens, Ponsaerts: "Plasmid-based genetic modification of human bone marrow-derived stromal cells: analysis of cell survival and transgene expression after transplantation in rat spinal cord." dans: BMC biotechnology, Vol. 7, pp. 90, (2008) (PubMed).

Williams, Weichert, Zakrzewicz, Da Silva-Azevedo, Pries, Baum, Egginton: "Differential gene and protein expression in abluminal sprouting and intraluminal splitting forms of angiogenesis." dans: Clinical science (London, England : 1979), Vol. 110, Issue 5, pp. 587-95, (2006) (PubMed).

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody