Chèvre anti-Souris IgG (Heavy & Light Chain) Anticorps (HRP) - Preadsorbed

N° du produit ABIN964951

Détail du produit

Antigène: IgG

Épitope: Heavy & Light Chain

Reactivité: Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)

Specificité: IgG (H&L)

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: immunoaffinity chromatography using Mouse IgG coupled to agarose beads

Immunogène: Mouse IgG whole molecule

Isotype: IgG

Information d'application

Indications d'application: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 2.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Gentamicin sulfate

Conseil sur la manipulation: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Référence

Metzner, Luckert, Lemuth, Hämmerle, Moos: "Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection." dans: Sensors (Basel, Switzerland), Vol. 18, Issue 5, (2018) (PubMed).

ONeill, Donohue, Omelchenko, Firestein: "The 3' UTRs of Brain-Derived Neurotrophic Factor Transcripts Differentially Regulate the Dendritic Arbor." dans: Frontiers in cellular neuroscience, Vol. 12, pp. 60, (2018) (PubMed).

Shrestha, Ahn, Staples, Sathyan, Karpova, Foltz, Basrai: "Mislocalization of centromeric histone H3 variant CENP-A contributes to chromosomal instability (CIN) in human cells." dans: Oncotarget, Vol. 8, Issue 29, pp. 46781-46800, (2018) (PubMed).

Castaño, Caviedes, Hirsch, Llanos, Iñiguez, Ronco: "Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels." dans: PLoS ONE, Vol. 12, Issue 1, pp. e0170389, (2017) (PubMed).

DLima, Ma, Winkler, Chu, Loh, Corpuz, Budnik, Lykke-Andersen, Saghatelian, Slavoff: "A human microprotein that interacts with the mRNA decapping complex." dans: Nature chemical biology, Vol. 13, Issue 2, pp. 174-180, (2016) (PubMed).

Ray, Chopra, Long, Lahiri: "Human primary mixed brain cultures: preparation, differentiation, characterization and application to neuroscience research." dans: Molecular brain, Vol. 7, pp. 63, (2015) (PubMed).

Hashimoto, Pais, Zhang, Saleh, Fu, Dai, Corrêa, Zheng, Cheng: "Structure of a Naegleria Tet-like dioxygenase in complex with 5-methylcytosine DNA." dans: Nature, Vol. 506, Issue 7488, pp. 391-5, (2014) (PubMed).

Frasca, Romero, Diaz, Alter-Wolf, Ratliff, Landin, Riley, Blomberg: "A molecular mechanism for TNF-α-mediated downregulation of B cell responses." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 1, pp. 279-86, (2012) (PubMed).

Frasca, Romero, Landin, Diaz, Riley, Blomberg: "Protein phosphatase 2A (PP2A) is increased in old murine B cells and mediates p38 MAPK/tristetraprolin dephosphorylation and E47 mRNA instability." dans: Mechanisms of ageing and development, Vol. 131, Issue 5, pp. 306-14, (2010) (PubMed).

Ronsyn, Daans, Spaepen, Chatterjee, Vermeulen, DHaese, Van Tendeloo, Van Marck, Ysebaert, Berneman, Jorens, Ponsaerts: "Plasmid-based genetic modification of human bone marrow-derived stromal cells: analysis of cell survival and transgene expression after transplantation in rat spinal cord." dans: BMC biotechnology, Vol. 7, pp. 90, (2008) (PubMed).

Williams, Weichert, Zakrzewicz, Da Silva-Azevedo, Pries, Baum, Egginton: "Differential gene and protein expression in abluminal sprouting and intraluminal splitting forms of angiogenesis." dans: Clinical science (London, England : 1979), Vol. 110, Issue 5, pp. 587-95, (2006) (PubMed).

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody