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Chèvre anti-Humain IgM (Fc5mu Region) Anticorps (FITC) - Preadsorbed

FACS, FM Polyclonal IgG FITC F(ab')2 fragment
N° du produit ABIN965101
  • Antigène Tous les produits IgM
    IgM
    Fragment
    F(ab')2 fragment
    Épitope
    • 162
    • 123
    • 87
    • 57
    • 25
    • 9
    • 7
    • 3
    • 1
    • 1
    Fc5mu Region
    Reactivité
    • 226
    • 159
    • 89
    • 42
    • 34
    • 34
    • 28
    • 24
    • 17
    • 14
    • 13
    • 13
    • 13
    • 12
    • 11
    • 4
    • 2
    • 1
    Humain
    Hôte
    • 364
    • 231
    • 89
    • 21
    • 14
    • 5
    • 4
    • 1
    • 1
    Chèvre
    Clonalité
    • 533
    • 95
    Polyclonal
    Conjugué
    • 122
    • 85
    • 84
    • 66
    • 34
    • 32
    • 26
    • 24
    • 14
    • 12
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 10
    • 9
    • 9
    • 9
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    FITC
    Application
    • 377
    • 282
    • 261
    • 221
    • 130
    • 124
    • 91
    • 91
    • 63
    • 62
    • 53
    • 53
    • 45
    • 38
    • 30
    • 20
    • 18
    • 12
    • 6
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Fluorescence Microscopy (FM)
    Specificité
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Human IgM and Human Serum.
    Attributs du produit
    This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Purification
    Preadsorption: Solid phase absorption
    Immunogène

    Immunogen: Human IgM Fc5mu  fragment

    Isotype
    IgG
    Labeling Ratio
    3.7
  • Indications d'application

    Application Note: F(ab')2 Anti-Human IgM Fc5μ Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Flow Cytometry Dilution: 1:500-1:2,500

    IF Microscopy Dilution: 1:500 - 1:2,500

    Commentaires

    Excitation/Emission wavelength: 494 nm/514 nm

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Product is photosensitive and should be protected from light.
    Avoid cycles of freezing and thawing.
    Do NOT add Sodium Azide!
    Stock
    RT,4 °C,-20 °C
    Stockage commentaire
    Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
    Date de péremption
    12 months
  • Antigène
    IgM
    Abstract
    IgM Produits
    Classe de substances
    Antibody
    Sujet

    Synonyms: Goat F(ab')2 Anti-Human IgM Fc5μ Antibody Fluorescein Conjugation, Goat Fab2 Anti-Human IgM Fc5μ FITC Conjugated Antibody

    Background: F(ab')2 Anti-Human IgM Fc5μ Fluorescein Antibody generated in goat detects specifically the Fc5μ portion of the human IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. F(ab')2 Anti-Human IgM Fc5μ antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

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