Chèvre anti-Humain IgG (Fc Region) Anticorps (HRP)

N° du produit ABIN965105

Détail du produit

Antigène: IgG

Fragment: F(ab')2 fragment

Épitope: Fc Region

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Western Blotting (WB)

Specificité: F(ab')2 Anti-Human IgG FcAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum.

Attributs du produit: F(ab')2 Anti-Human F(c) antibody was generated in goat and detects specifically Human IgG F(c) fragment. This secondary antibody anti-Human is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Purification: Immunoaffinity Chromatography.

Immunogène: Human IgG F(c) fragment
Immunogen Type: Native Protein

Information d'application

Indications d'application: F(ab') 2 Anti-Human Fc antibody is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Gentamicin sulfate

Conseil sur la manipulation: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Stock: 4 °C

Date de péremption: 12 months

Référence

Newby, Schiller, Wessler, Edelstein, Forest, Lai: "A blueprint for robust crosslinking of mobile species in biogels with weakly adhesive molecular anchors." dans: Nature communications, Vol. 8, Issue 1, pp. 833, (2018) (PubMed).

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet: Synonyms: F(ab')2 Human Antibody, Gt-a-Human Fc, Human IgG Fc antibody in Goat, F(ab')2 Human Secondary Antibody,