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Chèvre anti-Lapin IgG (F(ab')2 Region) Anticorps (Biotin) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Biotin F(ab')2 fragment
N° du produit ABIN965224
  • Antigène Tous les produits IgG
    IgG
    Fragment
    F(ab')2 fragment
    Épitope
    • 2382
    • 512
    • 423
    • 207
    • 132
    • 129
    • 95
    • 29
    • 28
    • 28
    • 22
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    F(ab')2 Region
    Reactivité
    • 724
    • 702
    • 603
    • 483
    • 403
    • 243
    • 212
    • 176
    • 147
    • 138
    • 113
    • 112
    • 107
    • 102
    • 84
    • 59
    • 48
    • 35
    • 28
    • 16
    • 12
    • 10
    • 8
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Lapin
    Hôte
    • 1791
    • 1576
    • 574
    • 244
    • 112
    • 111
    • 33
    • 31
    • 21
    • 19
    • 15
    • 11
    • 11
    • 1
    Chèvre
    Clonalité
    • 3450
    • 178
    • 1
    Polyclonal
    Conjugué
    • 589
    • 418
    • 417
    • 382
    • 282
    • 276
    • 244
    • 119
    • 108
    • 67
    • 66
    • 64
    • 61
    • 58
    • 52
    • 51
    • 48
    • 46
    • 45
    • 44
    • 41
    • 39
    • 36
    • 33
    • 33
    • 31
    • 30
    • 30
    • 29
    • 29
    • 29
    • 29
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 27
    • 26
    • 26
    • 25
    • 22
    • 21
    • 19
    • 17
    • 17
    • 16
    Biotin
    Application
    • 1955
    • 1853
    • 1809
    • 1521
    • 934
    • 910
    • 652
    • 478
    • 380
    • 236
    • 236
    • 234
    • 106
    • 103
    • 86
    • 67
    • 54
    • 53
    • 31
    • 28
    • 25
    • 21
    • 18
    • 16
    • 11
    • 9
    • 6
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificité
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
     Réactivité croisée
    Lapin
    Attributs du produit
    F(ab')2 anti-Rabbit IgG antibody generated in goat detects specifically Rabbit IgG whole molecule. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
    Purification
    Preadsorption: Solid phase absorption
    Immunogène

    Immunogen: Anti-Rabbit IgG F(ab')2 fragment was produced by repeated immunization with Rabbit IgG whole molecule in goat.

    Immunogen Type: Native Protein

    Isotype
    IgG
    Labeling Ratio
    10-20
  • Indications d'application

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: F(ab')2 anti-Rabbit IgG is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 500 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Aliquot to Avoid repeated freezing and thawing.
    Stock
    RT,4 °C,-20 °C
    Stockage commentaire
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
    Date de péremption
    12 months
  • Antigène
    IgG
    Abstract
    IgG Produits
    Classe de substances
    Antibody
    Sujet

    Synonyms: Goat F(ab')2 Anti-Rabbit IgG biotin conjugated Antibody, Goat Fab2 Anti-Rabbit IgG Antibody biotin Conjugation

    Background: F(ab')2 anti-Rabbit IgG antibody generated in goat detects specifically Rabbit IgG whole molecule. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

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