Lapin anti-Souris IgG (Heavy & Light Chain) Anticorps (TRITC) - Preadsorbed

N° du produit ABIN965362

Détail du produit

Antigène: IgG

Fragment: Fab fragment

Épitope: Heavy & Light Chain

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: TRITC

Application: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum.

Attributs du produit: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purification: Preadsorption: Solid phase absorption

Immunogène:

Immunogen: Mouse IgG whole molecule

Isotype: IgG

Labeling Ratio: 1.3

Information d'application

Indications d'application:

Application Note: This product is suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Conseil sur la manipulation: Avoid cycles of freezing and thawing.
Product is photosensitive and should be protected from light.
Centrifuge product if not completely clear after standing at room temperature.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet:

Synonyms: Rabbit Fab Anti-Mouse IgG Antibody Rhodamine Conjugation, Rabbit Fab Anti-Mouse IgG TRITC Conjugated Antibody

Background: Fab Anti-Mouse IgG (H&L) Rhodamine Antibody generated in rabbit detects Mouse IgG. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Fab Antibody is ideal for investigators who routinely perform flow cytometry, immunofluorescence, IHC, and other immunoassays. This Fab Anti-Mouse IgG Antibody is conjugated to rhodamine.