ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)
Specificité
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum.
Attributs du produit
Anti-Rabbit IgG whole molecule antibody generated in goat detects specifically Rabbit IgG whole molecule. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Rabbit IgG whole molecule antibody generated in goat detects specifically Rabbit IgG whole molecule. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Purification
Preadsorption: Solid phase absorption
Immunogène
Anti-Rabbit IgG whole molecule was produced by repeated immunization with Rabbit IgG whole molecule in goat. Immunogen Type: Native Protein
Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
Concentration
1.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Agent conservateur
Gentamicin sulfate
Conseil sur la manipulation
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Stock
4 °C
Stockage commentaire
Store vial at 4 °C before opening. This product is stable at 4 °C as an undiluted liquid.
Date de péremption
12 months
Lu, Stump, Seavey: "Novel method of monitoring trace cytokines and activated STAT molecules in the paws of arthritic mice using multiplex bead technology." dans: BMC immunology, Vol. 11, pp. 55, (2011) (PubMed).