Âne anti-Mouton IgG (Heavy & Light Chain) Anticorps - Preadsorbed
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- Antigène Tous les produits IgG
- IgG
- Fragment
- Fab fragment
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Épitope
- Heavy & Light Chain
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Reactivité
- Mouton
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Hôte
- Âne
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Clonalité
- Polyclonal
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificité
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum.
- Purification
- Preadsorption: Solid phase absorption
- Immunogène
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Immunogen: Sheep IgG whole molecule
- Isotype
- IgG
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- Indications d'application
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.9 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature.
- Stock
- 4 °C
- Stockage commentaire
- Store vial at 4 °C prior to opening. This product is stable at 4 °C as an undiluted liquid.
- Date de péremption
- 12 months
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- Antigène
- IgG
- Abstract
- IgG Produits
- Classe de substances
- Antibody
- Sujet
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Synonyms: Donkey Fab Anti-Sheep IgG Antibody, Donkey Fab Fragment Anti-Sheep IgG Antibody
Background: Fab Anti-Sheep IgG (H&L) Antibody generated in donkey detects immunoglobulin g from sheep, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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