Monoclonal Anti-Human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor
Purification
Antibodies are purified by protein A affinity chromatography
Western Blot: 1:200- 1:1,000 ELISA: Propose dilution 1:10,000. Determining optimal working dilutions by titration test.
Restrictions
For Research Use only
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Klonisch, Panayotou, Edwards, Jackson, Berger, Delves, Lund, Roitt: "Enhancement in antigen binding by a combination of synergy and antibody capture." dans: Immunology, Vol. 89, Issue 2, pp. 165-71, (1996) (PubMed).