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SPATA31D4 Protein (AA 1-917) (Strep Tag)

SPATA31D4 Origine: Humain Hôte: Tobacco (Nicotiana tabacum) Recombinant > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC). ELISA, SDS, WB
N° du produit ABIN3115897
  • Antigène
    SPATA31D4 (SPATA31 Subfamily D, Member 4 (SPATA31D4))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-917
    Origine
    Humain
    Source
    Tobacco (Nicotiana tabacum)
    Purification/Conjugué
    Cette SPATA31D4 protéine est marqué à la Strep Tag.
    Application
    ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Séquence
    MENILCFLNS YTETGLSPDS HCLDIDLNFI CLSGLGLFIL YLFYMVLTLY SSPTEKNNDT QKHQGRARRK RKSVTFKDRK SLQKEAEEER KLHSFLKSFG PPVSCSPLGQ HHDTTLFRRL LCPDPVCRVC NRATADIQRL LSWESLKDAA PSVSPLASSA SGAESSFTLA STPSATTPED LILSSRPKPS PPPPLILSPD LITTLADLFS PSPLRDPLPP QPVSPLDSKF PIDHSPPQQL PFPLLPPHHI ERVEPSLQPE ASLSLNTIFS FGSTLCQDIS QAVNRTDSCA RHHGPPTPSA LPPEDCTVTQ SKSNLTVLKT FPEMLSLGGS GGSSTSAPTT KGIDHSCPAS SEFSWWQPHA KDSFSSNFVP SDFMEELLTL HSSEASLGGH SVANIIQPVN ISFLSHDIPA LLERQVKRRG DFLMWKENGK KPGSFPTQLR PNYQLNSSRN MLTSTAVKHD LAESFPFWAS KGKLEWQHIH QQPPYSKCFE DHLEQKYVQL FWGLPSLHSE SLHPTVFVQH GRSSMFVFFN GITNTSMSHE SPVLPPPQPL FLPSTQPLPL PQTLPRGQSL HLTQVKSLAQ PQSPFPALPP SPLFLIRVCG VCFHRPQNEA RSLMPSEINH LEWNVLQKVQ ESVWGLPSVV QKSQEDFCPP APNPVLVRKS FKVHVPISII PGDFPLSSEV RKKLEQHIRK RLIQRRWGLP RRIHESLSLL RPQNKISELS VSESIHGPLN ISLVEGQRCN VLKKSASSFP RSFHERSSNM LSMENVGNYQ GCSQETAPKN HLLHDPETSS DEDLRSNSER DLGTHMMHLS GNDSGVRLGQ KQLENALTVH LSKKFEEINE GRMPGTVHSS WHSVKQTICL PEKSHSQIKH RNLAALVSED HGVDTSQEMS FLSSNKQKML EAHIKSFHMK PILNLSI
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Attributs du produit
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification
    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
    Pureté
    > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    SPATA31D4 (SPATA31 Subfamily D, Member 4 (SPATA31D4))
    Autre désignation
    SPATA31D4
    Synonymes
    FAM75D4 Protein, SPATA31 subfamily D member 4 Protein, SPATA31D4 Protein
    Sujet
    Spermatogenesis-associated protein 31D4 (Protein FAM75D4),FUNCTION: May play a role in spermatogenesis. {ECO:0000250}.
    Poids moléculaire
    102.3 kDa
    UniProt
    Q6ZUB0
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